Spec. Issue 2006 - Article Summary

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Standardisation in cell and tissue culture. The need for specific GLP guidelines in the cell culture laboratory (Good Cell Culture Practice – GCCP)

Gerhard Gstraunthaler
Department of Physiology and Medical Physics, Innsbruck Medical University, Innsbruck, Austria
The in vitro cultivation of eukaryotic cells has become a powerful technique in basic cell and molecular biological research, applied biotechnology, and in vitro alternatives. Before cell culture could be carried out successfully, two problems had to be overcome: (1) Populations of cells had to be established from single cells; and (2) these populations had to be maintained for many generations. In a successful propagation of cells in vitro, cells from various tissues should grow and proliferate under appropriate culture conditions, while preserving highly differentiated functions, which closely resemble their ancestor cells in vivo. For this purpose, the cell environment in vivo has to be mimicked in vitro. Thus, a number of selected culture conditions and methodological protocols have to be applied, which are handled quite differently in different tissue culture laboratories. Therefore, for good laboratory practice in the cell culture laboratory, well-defined and precisely described culture protocols are mandatory to ensure optimal and reproducible culture conditions, and to enable interlaboratory comparability of data and scientific results obtained with cultured cells.

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