Spec. Issue 2006 - Article Summary

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Ocular toxicology in vitro – cell based assays

Monica Berry and Marcus Radburn-Smith
Academic Unit of Ophthalmology, University of Bristol, UK
Interactions between the three cell types in the cornea control differentiation and responses to stimuli. We have sequentially added cell types in a 3-dimensional construct to assess the minimal requirements for a toxicology model of the human cornea. Co-cultured cell types displayed patterns of cytokines different from the single cell-type 3D models. Following exposure to toxicants there were marked changes in cytokine profiles, related to the toxicant and markedly influenced by the epithelial cell-line used. For a rational choice of cell lines, their steady-state patterns of signal molecules should be compared to those in normal human preocular fluid.

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