Spec. Issue 2006 - Article Summary

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Modelling long-term repeat-dose toxicity – challenges faced

Richard Clothier, Jonathan Crookdale, Jessica Morgan, Lucy Fitzgerald and Pauline Moore
FRAME Alternatives Laboratory, School of Biomedical Sciences, University of Nottingham, UK
Summary
Modelling of repeat/chronic-dose toxicity requires the in vitro maintenance of defined functions that are partly dependent on medium supplements. The ability to grow cells in the undifferentiated state and then control their differentiation is critical, since toxicity can affect differentiation triggers, differentiation control, and/or maintenance of the differentiated state.
Required monitoring endpoint assay methods should allow repeat exposure, and assess both functional and toxicological endpoints. In addition, in vitro toxicity should evaluate recovery from injury, since this is part of in vivo toxicity profiling.

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