Spec. Issue 2006 - Article Summary

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Effects of trichostatin A on apoptosis-regulating proteins during hepatocyte isolation

Tamara Vanhaecke, Mathieu Vinken, Tom Henkens, Sarah Snykers, Greetje Elaut, Peggy Papeleu and Vera Rogiers
Department of Toxicology, Vrije Universiteit Brussel, Brussels, Belgium
Summary
In this study, the expression levels of key apoptosis-regulating proteins were investigated by Western blot in primary rat hepatocytes during the various steps of their isolation in the presence and absence of trichostatin A (TSA). It was found that addition of 1 ┬ÁM TSA to the perfusate used for hepatocyte isolation reduced procaspase-3 cleavage as well as the expression of Apaf-1, the tumour suppressor p53, and the pro-apoptotic Bcl-2 proteins Bid and Bax. These findings indicate that, already during the isolation of primary hepatocytes from liver, TSA might direct cell signalling towards a p53-dependent reduction of apoptosis.

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