Spec. Issue 2006 - Article Summary

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Detecting neurotoxicity through electrical activity changes of neuronal networks on multielectrode neurochips

Alexandra Gramowski1, Simone Stüwe1, Konstantin Jügelt1, Dietmar Schiffmann1, Jan Loock2, Olaf Schröder3, Guenter W. Gross4 and Dieter G. Weiss1
1Institute of Biological Sciences, Department of Cell Biology and Biosystems Technology, 2Clinic for Internal Medicine, University of Rostock, Germany, 3Pattern Expert, Borsdorf, Germany, 4Center for Network Neuroscience, University of North Texas, Denton, TX, USA
The innovative alternative method of multielectrode neurochip recording, which offers improvements compared to standard animal experimentation, has been further optimised by advanced multi-parametric data analysis and used to study toxicity patterns of known toxicants. Neuronal networks on 64-electrode neurochips develop long-term stable spontaneous electrical activity. Changes in spatio-temporal electrical activity were quantified and subjected to pattern analysis to evaluate the effects of selected neurotoxic compounds. Several examples underline the suitability of this broadband biosensor system for functional neurotoxicity testing and safety pharmacology. Our results demonstrate that neuronal networks retain tissue-specificity and respond to transmitter receptor blockers and other neurotoxic compounds in a substance-specific, dose-dependent manner.

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