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Highly sensitive detection of cancer antigen 15-3 using novel avian IgY antibodies

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Renata Grzywa1, Agnieszka Łupicka-Słowik1, Maciej Walczak1, Magdalena Idzi1, Kamila Bobrek2, Stephane Boivin3, Andrzej Gaweł2, Tadeusz Stefaniak4, Józef Oleksyszyn1, and Marcin Sieńczyk1
1 Wroclaw University of Technology, Faculty of Chemistry, Division of Medicinal Chemistry and Microbiology, Wroclaw, Poland;
2 Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Department of Epizootiology and Clinic of Bird and Exotic Animals, Wroclaw, Poland;
3 European Molecular Biology Laboratory (EMBL), Hamburg, Germany;
4 Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, Wroclaw, Poland

Summary

Early detection of cancer development is crucial for successful therapy and for monitoring patient outcome. Various immunodiagnostic methods are able to detect pathological changes in the human body ahead of symptomatic manifestation of the disease. Most immunological examinations are based on the detection of specific tumor markers in body fluids. Of the various cancer-specific proteins used for breast cancer diagnostics, one of the most commonly applied is the cancer antigen 15-3 (CA 15-3). An elevation in its serum level (>25-40 U/ml) usually correlates with tumor malignancy. The CA 15-3 antigen is also used for monitoring patients after surgical treatment and for measuring therapeutic efficacy. Herein, we present the generation of polyclonal IgY antibodies isolated from egg yolks of immunized hens and their application for CA 15-3 detection. The developed sandwich ELISA assay showed a detection limit of 0.028 U/ml, thus demonstrating its potential for clinical applications.

 

Keywords: IgY antibody, breast cancer, CA 15-3 antigen, ELISA

 

 

ALTEX 31(1), 43–52

DOI: 10.14573/altex.1309181



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